The Assassination of Vasily Karpov was a failed attempt by Helmut Zemo to extract information about the Winter Soldier Program from former HYDRA agent Vasily Karpov.


Succeeding in acquiring the Super Soldier Serum, Karpov oversaw the creation of the Winter Soldiers, an elite group of five HYDRA assassins infused with the serum.

However, the Winter Soldiers, except for Barnes, lost control and started a riot, resulting in all of them being put under deep sleep in Cryostasis Chambers.